SDSU Rickettsias and Chlamydias Study Guide Questions
Description
1. Why are rickettsias and chlamydias not grown in laboratory cultures?
2. What is a serial dilution? Why would one use a serial dilution?
3. In a serial dilution where 1 ml of culture was plated from the 10-6 dilution, there were 253
colonies on the plate. What is the viable colony count of the organism?
4. What is the difference between a pour plate and a spread plate for quantifying bacteria?
5. Describe how each of the following would be used to measure bacterial growth:
a. Plate counts
b. Filtration
c. Turbidity using a spectrophotometer
d. Direct microscopic count
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