Psychology Question

Description

For this ELISA laboratory assignment, view each of the three videos below and answer the questions found at the end of these instructions.

ELISA – a test used to determine if a person is positive or negative to a particular virus, bacteria or other pathogen

Detects the presence of an antibody that was formed from exposure to the virus
Performing an ELISA involves at least one antibody with specificity for a particular antigen
In this test, a patient’s serum is diluted greatly (400 times) and placed on a plate on which HIV antigens are already present
If the serum contains antibodies for HIV, they stick to these antigens
The plate is then washed to remove all other components of patient’s serum
Another specially prepared antibody is applied to the plate
A substrate for enzyme is applied and the color changes because of catalysis of enzyme

ELISA Procedure

ELISA test procedureLinks to an external site. ELISA test procedure

The Enzyme Linked Immunosorbent Assay

The Enzyme Linked Immunosorbent Assay (ELISA)Links to an external site. The Enzyme Linked Immunosorbent Assay (ELISA)

Sample Laboratory Procedure – This video may need to be unmuted before viewing. It the playback is too fast to effectively listen to the instructions, go into the video settings and change the playback speed to 0.75

ELISA Testing-SARS CoV2 (COVID19)Links to an external site. ELISA Testing-SARS CoV2 (COVID19)

The following questions are to be answered during the viewing of the three videos. Questions are not placed in order of appearance.

ELISA Online Laboratory Questions and Answers

What do the letters, ELISA, stand for?
What is the purpose of PBS buffer used in the ELISA procedure?
Why are enzymes used in this immunoassay?
If the person is positive to the disease, do the specific or non-specific antibodies attach to the antigen during the ELISA procedure?
What is the purpose of the transfer pipettes used in the ELISA procedure?
Why is it necessary to assay positive and negative control samples as well as the person’s own experimental samples?
What happens to the proteins in the plastic well if the sample contains the antigen?
Why is it necessary to wash the wells after each ELISA step?
What is the purpose of the substrate used in the ELIZA?
Why is the incubator used in the ELIZA?

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