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Harvard University Gene Cloning Presentation

Harvard University Gene Cloning Presentation

Description

Slide 5: Creating a Clone: DNA purification and bacterial transformation

  • Why is DNA purification by gel electrophoresis needed?
  • How can cells be made competent?
    • Electroporation
    • Heat shock

Slide 6: Gene Clone verification

  • Gel electrophoresis to estimate band size
  • DNA sequencing

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