Name	Course	Date
Andre Woods	BIOL 103	11/14/22

Activity Data Code WRC

Procedure I – Enzyme Reaction Rate – Concentration Dependence

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Trial	Concentration Level	Oxygen Concentration Change Data (ppt)	Elapsed Time (s)	Enzyme Reaction Rate (ppt/s)
1	2.5%	4.70	20.0	0.235
2	5.0%	9.40	20.0	0.47
3	10%	18.80	20.0	0.94

Observations and Questions

[1] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.

Enzyme reaction rate = 4.70 ppt / 20.0 s = 0.235 ppt/s

[2] Convert enzyme concentrations of 2.5%, 5.0%, and 10% to ppt units.

1% = 1/100 1ppt = 1/1000 then 1% = 10 ppt

2.5% = 25.0 ppt

5.0% = 50.0 ppt

10% = 100.0 ppt

[3] For Procedure I/Table I, identify:

Independent variable:

Dependent variable:

Control variables:

[4] Draw a graph of the independent variable on the x-axis (abscissa) and the dependent variable on the y-axis (ordinate). Label the axis titles and units.

Procedure II – Enzyme Reaction Rate – Temperature Dependence

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Trial	Temperature (°C)	Oxygen Concentration Change Data (ppt)	Elapsed Time (s)	Enzyme Reaction Rate (ppt/s)
1	20.0	6.63	20.0	0.3315
2	30.0	13.34	20.0	0.667
3	55.0	2.08	20.0	0.104

Observations and Questions

[5] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.

Enzyme reaction rate = 6.63 ppt / 20.0 s = 0.3315 ppt/s

[6] Using the data you reported in the Table above, describe the effect of temperature on the reaction rate.

The effect of temperature on the reaction rate steadily increased from trial one’s 20.0 up to trial 3 55.0. Between trial 1 and 2 the reaction rate basically doubled.

Procedure III – Enzyme Reaction Rate – pH Dependence

Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Trial	pH Level	Oxygen Concentration Change Data (ppt)	Elapsed Time (s)	Enzyme Reaction Rate (ppt/s)
1	6.0	5.71	20.0	0.2855
2	7.0	9.40	20.0	0.47
3	9.0	1.27	20.0	0.0635

Observations and Questions

[7] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.

Enzyme reaction rate = 5.71 ppt / 20.0 s = 0.2855 ppt/s

[8] Choose either a bar graph or a line graph format and plot the data for Procedures II and III. Plot the data for each Procedure on a separate plot.

[9] Compare the plot of the data for Procedures I, II, and III. Describe the differences among the three plots.

[10] What conclusions can draw about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?

[11] Choose one of the factors that impacts reaction rate (concentration, temperature, or pH) and, in your own words, provide a plausible biological explanation for your experimental results for that factor.