San Diego State Importance of Genetics in Contemporary Laboratories Questions

Description

Molecular biology techniques are being increasingly used in diagnostic laboratories. The polymerase chain reaction (PCR) is one of the newest techniques available and potentially offers tremendous advantages for the diagnosis of infectious diseases.

DNA SEQUENCINGWITH PCR

Read the file withthe back ground information

Then read this account at

http://www.wiley.com/legacy/college/boyer/0470003790/animations/pcr/pcr.htm

Complete the critical Thinking writing assignments

1.It has been suggested that the triplet genetic code evolved from a two-nucleotide code. Perhaps there were fewer amino acids in the ancient proteins. Comment on the features of the genetic code that might support this hypothesis?

2.The strands of DNA can be separated by heating the DNA sample. The input heat energy breaks the hydrogen bonds between base pairs, allowing the strands to separate from one another. Suppose that you are given two DNA samples. One has a G + C content of 70% and the other has a G + C content of 45%. Which of these samples will require a higher temperature to separate the strands?Explain your answer.

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